2019006133

CERTIFICATE OF ANALYSIS
QUALITY CONTROL PERFORMANCE REPORT

~~COLUMN~~PRODUCT INFORMATION

SERIAL NUMBER : 2019006133

PART NUMBER : S8034-034360
PRODUCT TYPE : ChraSep SPE Columns ODS-IC 500mg/3ml, 50/pk
LOT : 639753201

RAW MATERIALS

Component Properties

Properties

Specifications

Results

Methods

Tube Purity

Proprietary

Pass

GC FID Test

Frit Purity

Proprietary

Pass

GC FID Test

Base Silica Physical Properties

Properties

Specifications

Results

Methods

Specific Surface Area (m²/g)

450-550

535

N2 BET

Average Particle Size (µm)

45-60

Malvern 100

Average Pore Diameter (Å)

60-90

N2 BET

Particle Size Distribution (%)

≤20µm ≤ 35µm ≥80µm

Malvern 100

≤ 11 ≤18 ≤15

≤ 8 ≤15 ≤10

PRODUCT SPECIFICATION/ANALYSIS

Bonded Silica Properties

Properties

Specifications

Results

Methods

Carbon Loading (%C)

17.0-20.0

19.5

CHNO-S Analysis

Hydrogen Loading(%H)

1.5-6.0

3.95

CHNO-S Analysis

Surface Coverage (µmol/m²)

1.90-2.50

2.05

Calculated based on %C

Turbidity (NTU)

≤10.0

0.55

Turbidity meter

Washable Residue (mg/g)

≤2.0

0.2

Methanol and Hexane gravimetric

Bed Mass Consistency

Proprietary

Pass

Weight Measurement

Flow Characteristics

Proprietary

Pass

Methanol in low vacuum (1” Hg)

HPLC Test Conditions

Column ~~Dimension:~~Dimension

100 x 4.6 ~~mm,~~mm

Flow

1 mL/min

Detector

UV@254 nm

Mobile Phase

Methanol:Water (60:40)

Temperature

25-35 oC
~~Probes:~~^oC

Probes

Uracil, Acetophenone, Nitrobenzene, Benzene, Toluene

MATERIAL SAFETY DATA SHEETS ARE AVAILABLE UPON REQUEST

ChraReport ~~at https://chrareport.com/2019006133~~

~~RAW MATERIALS~~
~~Component Properties~~
~~Properties~~	~~Specifications~~	~~Results~~	~~Methods~~
~~Tube Purity~~	~~Proprietary~~	~~Pass~~	~~GC FID Test~~
~~Frit Purity~~	~~Proprietary~~	~~Pass~~	~~GC FID Test~~
~~Base Silica Physical Properties~~
~~Properties~~	~~Specifications~~	~~Results~~	~~Methods~~
~~Specific Surface Area (m~~²~~/g)~~	~~450-550~~	~~535~~	~~N2 BET~~
~~Average Particle Size (µm)~~	~~45-60~~	46	~~Malvern 100~~
~~Average Pore Diameter (Å)~~	~~60-90~~		~~N2 BET~~
~~Particle Size Distribution (%)~~	≤~~20um ≤ 35um ≥80um~~ ≤ ~~11 ≤18 ≤15~~	≤~~20um ≤ 35um ≥80um~~ ≤ ~~8 ≤15 ≤10~~	~~Malvern 100~~
~~PRODUCT SPECIFICATION/ANALYSIS~~
~~Bonded Silica Properties: ChraSep SPE Columns ODS-IC 40UM~~
~~Properties~~	~~Specifications~~	~~Results~~	~~Methods~~
~~Carbon Loading (%C)~~	~~17.0-20.0~~	~~19.5~~	~~CHNO-S Analysis~~
~~Hydrogen Loading(%H)~~	~~1.5-6.0~~	~~3.95~~	~~CHNO-S Analysis~~
~~Surface Coverage (µmol/m~~²)	~~1.90-2.50~~	~~2.05~~	~~Calculated based on %C~~
~~Turbidity (NTU)~~	≤~~10.0~~	~~0.55~~	~~Turbidity meter~~
~~Washable Residue (mg/g)~~	≤~~2.0~~	~~0.2~~	~~Methanol and Hexane gravimetric~~
~~Bed Mass Consistency~~	~~Proprietary~~	~~Pass~~	~~Weight Measurement~~
~~Flow Characteristics~~	~~Proprietary~~	~~Pass~~	~~Methanol in low vacuum (1” Hg)~~

~~ChraReport~~ at https://chrareport.com/2019006133