2019006133

LC COLUMN
QUALITY CONTROL PERFORMANCE REPORT

COLUMN INFORMATION

SERIAL NUMBER : ~~2002000174~~2019006133

PART NUMBER : ~~C1505-B5034~~S8034-034360
~~COLUMN~~PRODUCT TYPE : ~~ChraSil~~ChraSep ~~B100~~SPE ~~C18,~~Columns ~~250~~ODS-IC 500mg/3ml, 50/pk
LOT : 639753201

HPLC Test Conditions
Column Dimension: 100 x 4.6 ~~mm,~~mm
Flow ~~5.0um~~
~~LOT~~1 ~~: KL0051~~

~~TEST CONDITIONS~~
~~MOBILE PHASE : 80% Acetonitrile / 20% water~~
~~COLUMN PRESSURE : 67Bar~~
~~COLUMN FLOW : 1.00 ml /~~ mL/min
~~LINEAR~~Detector ~~VELOCITY~~UV@254 :nm
Mobile ~~0.205~~Phase ~~cm / sec~~
~~TEMPERATURE : AMBIENT~~Methanol:Water (~60:40)
Temperature 2325-35 0C)oC
~~INJECTTION~~Probes: ~~VOLUME~~ Uracil, :Acetophenone, ~~0.5~~Nitrobenzene, µl

~~TEST VALUES SPECIFICATIONS~~
~~THEORETICAL PLATES : 22948 MIN : 91793~~
~~SELECTIVITY : 3.27 RANGE : 3.02 - 3.62~~
~~USP TAILING FACTOR : 1.01 RANGE : 0.98 - 1.25~~
~~(@ 5% Peak Height) k’ : 2.21~~

~~Peak Conc Sample (µg/ml) Component~~
~~1. 5 Uracil~~
~~2. 200 Phenol~~
~~3. 25 4-Chloro Nitrobenzene~~
~~4. 850~~ Benzene, Toluene

MATERIAL SAFETY DATA SHEETS ARE AVAILABLE UPON REQUEST

ChraReport at https://chrareport.com/2002000174

RAW MATERIALS
Component Properties
Properties	Specifications	Results	Methods
Tube Purity	Proprietary	Pass	GC FID Test
Frit Purity	Proprietary	Pass	GC FID Test
Base Silica Physical Properties
Properties	Specifications	Results	Methods
Specific Surface Area (m²/g)	450-550	535	N2 BET
Average Particle Size (µm)	45-60	46	Malvern 100
Average Pore Diameter (Å)	60-90		N2 BET
Particle Size Distribution (%)	≤20µm ≤ 35µm ≥80µm ≤ 11 ≤18 ≤15	≤20µm ≤ 35µm ≥80µm ≤ 8 ≤15 ≤10	Malvern 100

PRODUCT SPECIFICATION/ANALYSIS
Bonded Silica Properties: BOND SI 40UM C18 TOLUENCE
Properties	Specifications	Results	Methods
Carbon Loading (%C)	17.0-20.0	19.5	CHNO-S Analysis
Hydrogen Loading(%H)	1.5-6.0	3.95	CHNO-S Analysis
Surface Coverage (µmol/m²)	1.90-2.50	2.05	Calculated based on %C
Turbidity (NTU)	≤10.0	0.55	Turbidity meter
Washable Residue (mg/g)	≤2.0	0.2	Methanol and Hexane gravimetric
Bed Mass Consistency	Proprietary	Pass	Weight Measurement
Flow Characteristics	Proprietary	Pass	Methanol in low vacuum (1” Hg)