C1277-0518

Columns             : ChraSil B100 C8 150mm x 4.6mm, 5 µm

Mobile Phase    : A: H₂O + 0.1% HCOOH,

                           B: CH₃CN + 0.1% HCOOH

Gradient              : 30% - 40% B in 15 mins, hold at 40% B for 15 mins

Flow Rate            : 1.0 mL/min

Temperature        : Ambient

Detection            : UV, 254 nm

Peak Identification

1. . 7-Aminoclonazepam

2. . 7-Aminoflunitrazepam

3. . Nordoxepin

4. . Doxepin

5. . Desipramine

6. . Imipramine

7. . Nortriptyline

8. Amitriptyline
9. Trimipramine
10. Clomipramine
11. Nordiazepam
12. Clonazepam
13. Flunitrazepam
14. Diazepam