# C2087-0509

Determination of Caffeine Metabolites by HPLC-UV together with ChraSil B100 C8, 150mm x 4.6mm, 5µm

<table id="bkmrk-column%C2%A0-%C2%A0-%C2%A0-%C2%A0-%C2%A0-%C2%A0-%C2%A0%3A" style="height: 68px;" width="839"><tbody><tr><td style="width: 435.538px;">Column : ChraSil B100 C8, 150mm x 4.6mm, 5µm

Part No. : [C1515-B5032](https://chrasil.com/C1515-B5032 "C1515-B5032")

Mobile Phase : CH<sub>3</sub>OH:1% CH<sub>3</sub>COOH in H<sub>2</sub>O - 10:90

Flow Rate : 1.0 mL/min

Temperature : Ambient

Detection : UV, 254 nm</td><td style="width: 373.351px;">**Peak Identification**

1. 7-Methylxanthine
2. 1-Methyluric acid
3. 3-Methylxanthine
4. 1-Methylxanthine
5. 3,7-Dimethylxanthine
6. 1,7-Dimethylxanthine
7. 1,3-Dimethylxanthine

</td></tr><tr><td colspan="2" style="width: 808.889px;">[![image-1627385270539.png](https://chratec.info/uploads/images/gallery/2021-07/scaled-1680-/image-1627385270539.png)](https://chratec.info/uploads/images/gallery/2021-07/image-1627385270539.png)

 ChraNotes

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