# C2086-0508

Determination of Antihistamines by HPLC-UV together with ChraSil D120 C8,150mm x 4.6mm, 5µm

<table id="bkmrk-column%C2%A0-%C2%A0-%C2%A0-%C2%A0-%C2%A0-%C2%A0-%C2%A0%3A" style="height: 824px;" width="893"><tbody><tr style="height: 209px;"><td style="width: 435.521px; height: 209px;">Column : ChraSil D120 C8, 150mm x 4.6mm, 5µm

Part No. : [C1440-D5032](https://chrasil.com/C1440-D5032 "C1440-D5032")

Mobile Phase : CH<sub>3</sub>OH:5 mM NH<sub>4</sub>HCO<sub>3</sub>,

 pH10 - 75:25

Flow Rate : 1.0 mL/min

Temperature : Ambient

Detection : UV, 254 nm</td><td style="width: 373.368px; height: 209px;">**Peak Identification**

1. Pheniramine
2. Doxylamine
3. Pyrilamine
4. Phenothiazine
5. Diphenhydramine
6. Loratadine
7. Promethazine
8. Chlocylizine

</td></tr><tr style="height: 615px;"><td colspan="2" style="width: 808.889px; height: 615px;">[![image-1627384964558.png](https://chratec.info/uploads/images/gallery/2021-07/scaled-1680-/image-1627384964558.png)](https://chratec.info/uploads/images/gallery/2021-07/image-1627384964558.png)

 ChraNotes

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