# C2085-0507

Determination of Water Soluble Vitamins by HPLC-UV together with ChraSil D120 Phenyl, 150mm x 4.6mm, 5µm

<table id="bkmrk-column%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0-%3A-c" style="height: 88px;" width="844"><tbody><tr><td style="width: 435.538px;">Column : ChraSil D120 Phenyl, 150mm x 4.6mm, 5µm

Part No. : [C1448-D5032](https://chrasil.com/C1448-D5032 "C1448-D5032")

Mobile Phase : A: 10 mM HCOONH<sub>4</sub>, pH 3.0,

 B: CH<sub>3</sub>OH

 A:B - 95:5

Flow Rate : 1.0 mL/min

Temperature : Ambient

Detection : UV, 214 nm

</td><td style="width: 373.351px;">**Peak Identification**

1. Pyridoxal
2. Pyridoxine
3. Niacinamide

</td></tr><tr><td colspan="2" style="width: 808.889px;">[![image-1627382225180.png](https://chratec.info/uploads/images/gallery/2021-07/scaled-1680-/image-1627382225180.png)](https://chratec.info/uploads/images/gallery/2021-07/image-1627382225180.png)

 ChraNotes

</td></tr></tbody></table>