# C2075-0517

 Determination of Tricyclic Antidepressants via LC-UV using ChraSil B100 C18, 150mm x 4.6mm, 5µm

<table id="bkmrk-column%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0" style="width: 862px; height: 630px;"><tbody><tr><td style="width: 431.285px;">Column : ChraSil B100 C18, 150mm x 4.6mm, 5µm

Part No. : [C1505-B5032](https://chrasil.com/C1505-B5032 "C1505-B5032")

Mobile Phase : A: H<sub>2</sub>O + 0.1% HCOOH,

 B: CH<sub>3</sub>CN + 0.1% HCOOH

Gradient : 30% - 40% B in 15 minutes

Flow Rate : 1.0 mL/min

Temperature : Ambient

Detection : UV, 254 nm

</td><td style="width: 377.604px;">**Peak Identification**

1. Nordoxepin
2. Doxepin
3. Desipramine
4. Imipramine
5. Nortriptyline
6. Amitriptyline
7. Trimipramine
8. Clomipramine

</td></tr><tr><td colspan="2" style="width: 808.889px;">[![image-1627371932648.png](https://chratec.info/uploads/images/gallery/2021-07/scaled-1680-/image-1627371932648.png)](https://chratec.info/uploads/images/gallery/2021-07/image-1627371932648.png)

ChraNotes at https://chranote.com/C2075-0517

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